English: ISMA time-lapse movie of multiple lumen formation. This experiment also followed the experimental design described in the text and used Table 2 parameter settings. Cell polarization begins at 0:08 and lumen formation occurs at 0:09. (Note cells without lumen contact near the top of the cyst from 0:09 to 0:34.) Second and third lumens form at 0:35 and 0:36, delaying stabilization of neighboring cells. Most cells stabilize at 0:42, while cells not in contact with primary lumen stabilize at 0:44. Lumens merge at 1:08 and 1:09. Frame rate: 6 simulation cycles per second.