File:Rap1-Can-Bypass-the-FAK-Src-Paxillin-Cascade-to-Induce-Cell-Spreading-and-Focal-Adhesion-Formation-pone.0050072.s006.ogv

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English: Representative automated detection of 007-induced cell spreading. A549-Epac1 cells expressing GFP-Lifeact were trypsinised and allowed to roll for 1 hour before plating out onto fibronectin in the presence of 100 µM 007. Filming began approximately 30 minutes after cells were first applied to the fibronectin. Images were captured every 5 minutes through the 63×objective, and the movie rate is 7 frames per second. The scale bar represents 10 µm. Detection of cells was performed by local watershed segmentation upon smoothing (the detected cell edge is shown in red).
Date
Source Movie S4 from Ross S, Spanjaard E, Post A, Vliem M, Kristyanto H, Bos J, de Rooij J (2012). "Rap1 Can Bypass the FAK-Src-Paxillin Cascade to Induce Cell Spreading and Focal Adhesion Formation". PLOS ONE. DOI:10.1371/journal.pone.0050072. PMID 23209645. PMC: 3507925.
Author Ross S, Spanjaard E, Post A, Vliem M, Kristyanto H, Bos J, de Rooij J
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