English: A. Representative images from a typical intravital imaging Z-stack with H2B-Apple labeled nuclei (top row) and segmented regions identified from a negative of the original image using the described segmentation algorithm (bottom row; green outlines depict the segmented cell regions detected by the algorithm in each Z-slice). All scale bars represent 50 µm. B. Orthogonal views of the 3D Z-stack displaying segmented cell region outlines (green) in each view. C. Summation of the Z-stack containing all combined segmented region outlines (green).